As IRAP-pHluorin has been considered a reporter for GLUT4 trafficking [26], these findings suggest that these CNM-causing mutations might impair GLUT4 surface expression in skeletal muscle cells, as observed in myofibers of knock-in mice and biopsies from CNM patients, both carrying the dynamin-2 p.R465W mutation [21]. This evidence concerns the gene SLC2A4 and centronuclear myopathy.