The results showed that HRG quenches the S100A8/A9 binding to the surfaces of both B16-BL6 mouse melanoma cells (Figure 2A) and A375 human melanoma cells (Figure 2B), suggesting that S100A8/A9 loses its binding force to cell surface SSSRs via the formation of a complex with HRG. This evidence concerns the gene S100A8 and melanoma.