The mRNA (messenger ribonucleic acid) expression levels of SIRT1, FoxO1 and FoxO3a were significantly decreased in 4TLM metastatic tumors compared to 67NR non-metastatic tumors, whereas the FoxO4 signal was significantly increased in metastatic tumors (Figure 2e) (p ˂ 0.05). This evidence concerns the gene FOXO4 and metastatic neoplasm.