Since TIM-1 has been implicated in the regulation of endothelial function at the level of the blood–brain barrier (BBB), and its levels have been shown to be associated with stroke and cerebral ischemia-reperfusion injury [21,40], the main aim of this study was to identify and validate miRNAs that specifically target TIM-1 in human brain microvascular endothelial cells (hBMECs). This evidence concerns the gene HAVCR1 and Cerebral ischemia.