For example, osteosarcoma cell lines exposed to doses of 8 ppm of NaF showed alterations in the expression of genes associated with bone formation [62]; another study in male mice showed gaps and fissures of chromatids in the metaphase stage after exposition to high fluorine concentrations (4–20 mg/L) [63]; additionally, when the cell was exposed to concentrations of up to 5 mM of sodium fluoride, phosphorylation of the histone H2AX of LS8 ameloblast DNA was observed, which can induce cell apoptosis [64,65]. This evidence concerns the gene H2AX and osteosarcoma.