The fact that we observed only six patients with AKI and extended our investigation with animals samples could be a limitation of this study, but we also want to underline that the PAX8 expression pattern in human samples of myoglobin cast AKI was very similar to the PAX8 expression in the animal experimental model of postischemic acute kidney injury, so we can say that the results obtained from our research are comparable and promising in terms of determining renal PAX8 expression and might have prognostic significance in acute and chronic kidney diseases. Here, PAX8 is linked to chronic kidney disease.