Following the in vivo infection of rats by H. pylori and treatment with PEAME alone or with metronidazole as detailed in the experimental section, some criteria were assessed including the effect on the weight of the rats, colonization of H. pylori in the stomach tissue of rats, lipid peroxidation markers, gene expression of TNF-α and autophagy genes in the stomach tissue of rats, histopathological investigations and assessing the level of COX2 in the stomach tissues. Here, TNF is linked to infection.