As the first step in identifying novel GLI transcriptional targets that are in crosstalk with MAPK or other signaling pathways dysregulated in melanoma and that could be considered for combination therapy, we performed RNA sequencing on melanoma cell lines with overexpressed GLI1, GLI2, and GLI3 (Figure 2A and Supplementary Figure S3 show MDS plots). This evidence concerns the gene GLI1 and melanoma.