Furthermore, besides FISH analysis, there are currently several other methods to detect ALT status as for example terminal restriction fragment (TRF) Southern-blot analysis of telomere length profile, combined promyelocytic leukemia (PML) immunofluorescence/telomere FISH analysis of tumor sections for ALT associated PML or using C-circle amplification assay [88]. The gene discussed is PML; the disease is acute promyelocytic leukemia.