PTEN and neoplasm: The initial validation of bioinformatic data was carried out in both tumor cell lines and included the evaluation of KRAS, PTEN, TIMP3, PDCD4, MMP9, STAT3 and ADAM17 mRNA levels by qPCR, quantification of Pten and Stat3 protein levels by Western blot analysis, and measurement of Mmp9 protein level by flow cytometry (Figure 9, Figure 10 and Figure S12).