ATAD2 and breast cancer: Accordingly, Western blot analysis showed that the ATAD2 protein was not detected in MCF-10A, while it was slightly expressed in two breast cancer cell lines (HS578T and MDA-MB-468) and highly expressed in five breast cancer cell lines (MCF-7, MDA-MB-231, MDA-MB-436, SKBR3, and T47D) (Figure 1E).