As shown in Figure 2A, the addition of hPD-1 tumor-infiltrating CD3+CD8+ T cells in hPD-L1 MC38 cells remarkably decreased hPD-L1 MC38 cell viability on 60% of reductions, which was higher than that of hPD-L1 MC38 cells alone and greater than that of the co-culture with hPD-1 tumor-infiltrating CD3+ T cells. This evidence concerns the gene CD8A and neoplasm.