THBS1 and hepatocellular carcinoma: Moreover, mutations of the three different predicted canonical and non-canonical seed sequences (binding sites for miR-22) in the TSP1 3′-UTR of the pLightSwitch-3UTR-TSP1 vector further validated the functional relevance of only the first seed sequence in the 3′-UTR of TSP1 (WT site 1), over the three miR-22 seed sequences predicted by the miRWalk2.0 database, as a direct binding site for miR-22 in hepatoma cells (Figure 6F and Figure S7).