Given the strong ZNF365 signal in alveolar epithelial cells in IPF lung tissues, we also measured its expression in the A549 epithelial cell line by qPCR and WB, corroborating that ZNF365 is upregulated in these cells by the effect of TGFβ-1. The gene discussed is TGFB1; the disease is idiopathic pulmonary fibrosis.