To test the intervention effect of PGC-1α on the variants of mitochondrial fusion/fission proteins in AD, N2A cells were co-transfected with APPswe and pEnCMV/Pgc-1alpha plasmid for 48 h, and GFP-labeled APPswe and Flag-labeled PGC-1α were examined to confirm the success of transfection (Figure 7A). Here, PPARGC1A is linked to Alzheimer disease.