Next, to investigate whether AYC-EVs inhibit the OVA-induced DC maturation in asthma models, single cell suspensions of splenocytes for all groups were stained with lineage cocktails, and anti-CD11c, anti-CD80, anti-MHC-I, and anti-MHC-II antibodies, followed by the analysis of the matured splenic DCs (CD80 and MHC-I expression on lineage-CD11c+MHC-II+ splenic DCs) using a gating strategy for flow cytometry, as shown in Figure 7a. The gene discussed is ITGAX; the disease is asthma.