From the point of view of virulence gene analysis, although the Bartha K61 vaccine strain carries deletions encoding gI, gE, US9, and US2 genes [23,29], in which gE and gI jointly affect virulence [30] and are necessary for the anterograde neural transport of virus particles [31], some studies have found that the PRV Bartha K61 strain can still cause death of mice at 9 days (220 h) after infecting both sides of mouse skin [25], and KM mice inoculated with 105 TCID50 Bartha K61 developed clinical signs of disease that included pruritus and clawing and biting the injection site at 96 hpi. Here, GNAI1 is linked to Pruritus.