RPS16 and Pruritus: From the point of view of virulence gene analysis, although the Bartha K61 vaccine strain carries deletions encoding gI, gE, US9, and US2 genes [23,29], in which gE and gI jointly affect virulence [30] and are necessary for the anterograde neural transport of virus particles [31], some studies have found that the PRV Bartha K61 strain can still cause death of mice at 9 days (220 h) after infecting both sides of mouse skin [25], and KM mice inoculated with 105 TCID50 Bartha K61 developed clinical signs of disease that included pruritus and clawing and biting the injection site at 96 hpi.