ROC curve analysis was performed to discriminate between the groups with pre-DM and T2DM; the optimum cutoff values of ZBP1, HSPA1B, TMEM173, DDX58, NFKB1 and CHUK were 15.590, 6.898, 12.751, 26.5700, 17.350 and 17.740 with sensitivity of 75.4%, 85.2%, 90.2%, 85.2%, 90.2%, 73.8%, respectively, as shown in Figure 3A–F. The gene discussed is NFKB1; the disease is diabetes mellitus.