The black module was found to be enriched with microglial marker genes and AD-related signature genes, such as SPP1, CD163, CX3CR1, ETS1, CEBPB, STAT3, SPI1, NFATC2, DOCK8, RUNX1, ETV6, etc. Surprisingly, these genes were also enriched in the MG1 cluster in snRNA-seq data. Here, SPP1 is linked to Alzheimer disease.