Finally, 16 potential targets relative to depression were acquired from the TCMSP database (https://old.tcmsp-e.com/disease.php?qd=228, accessed on 19 March 2022) and further analyzed by the online STRING database (http://string.embl.de/, accessed on 15 March 2022) to explore the functional mechanism of the NUCB1 protein. This evidence concerns the gene NUCB1 and major depressive disorder.