Our results showed that total glutathione and the GSH/GSSG ratio of the soleus and EDL muscle (Figure 4) were decreased during obesity, suggesting that obesity interferes with the synthesis of this antioxidant, possibly because the ROS that are overproduced during this disease are related in the activation of signaling pathways that adversely affect glutathione synthesis such as NF-Kb, Sp1, E [11,23,50]. The gene discussed is SP1; the disease is obesity due to melanocortin 4 receptor deficiency.