In the present research, higher expression of TNF-α and IL-1β in both systemic and mucosal tissues was induced in the BI-vaccinated groups against S. iniae and A. hydrophila infection, respectively, illustrating that BI-immunized fish were more effective in activating macrophage and phagocyte activity against bacterial infection compared to the control group [1]. The gene discussed is IL1B; the disease is bacterial infectious disease.