Our data suggest that H3.3G34W may regulate the expression of SCUBE3 by altering the balance between H3K27ac and H3K27me3 at its proximal enhancer region: these data are in keeping with recent work which shows a gain in H3K27me3 in G34R-mutated brain tumours [21] as well as in GCT cell lines [12]. This evidence concerns the gene SCUBE3 and brain neoplasm.