In pleural mesothelioma, Group 1 had significantly lower TP53 alterations (prevalence = 9.0%, χ2 = 11.7, P value = 4.2 × 10−3) while Group 4 was characterised by a significantly higher prevalence of TP53 (prevalence = 30.3%, χ2 = 31.8, P value = 1.5 × 10−7) and RB1 alterations (prevalence = 4.3%, χ2 = 11.2, P value = 5.0 × 10−3) compared to the entire pleural mesothelioma group. This evidence concerns the gene RB1 and pleural mesothelioma.