PDCD1LG2 and neoplasm: Conventional flow cytometry gating confirmed these observations (Supplementary Table 1, Supplementary Figs. 1 and 2) and a comparison of FAK-wt and FAK−/− tumours identified that FAK-depletion results in a downregulation of PD-L2 expression on tumour-associated macrophages, endothelial cells, CD11b+ DCs and CD11b− DCs (Fig. 1f).