FREM1 and Zinc deficiency: However, marginal zinc deficiency significantly increased (p < 0.05) the relative expression of genes involved in LT (eltA and eltB), ST (estB), motility (motA), cellular adhesion (faeG), and biofilm formation (bssS) in mice with ETEC infection, though no significant differences (p > 0.05) were found in the relative expression of genes involved in quorum sensing (luxS) (Figure 10a–h).