The identified four protein kinases interact with cellular kinase-like cyclin-dependent kinase 1 (CDK1) or transcription factors, such as E2F, receptor proteins, such as breast cancer anti-estrogen resistance protein 1 (BCAR1), filamentous proteins, such as ACTA1, filamin-A (FLNA), vimentin (VIM), and F-actin-capping protein subunit α-1 (CAPZA1). This evidence concerns the gene ACTA1 and breast cancer.