CYBB and diabetes mellitus: Increased levels of the glycolytic intermediate glyceraldehyde-3-phosphate, after reduction to glycerol-3-phosphate, can lead to de novo generation of diacylglycerol, particularly in the context of elevated free fatty acids; diacylglycerol, via activation of protein kinase C, can promote assembly and activation of NOX2-dependent NAPDH oxidase; this mechanism is thought to be largely responsible for elevated NADPH oxidase activity in diabetes [4,185,186,187].