CD274 and cancer: KEGG pathway analysis of DEG genes was also performed, and it was revealed that the pathways that had the highest enrichment scores for the DEG genes were riboflavin metabolism (0.71), histidine metabolism (0.64), antigen processing and presentation (0.6), Toll-like receptor signaling, (0.6), circadian rhythm (0.57), lysosome (0.55), calcium signaling and the PD-L1 expression/PD-1 checkpoint pathway in cancer (Figure 4B,C).