Compared with the cerebral ischemia-reperfusion group, the infarct volume significantly decreased in the 25 mg/kg TSN I intervention group, the number of NeuN-positive neurons was markedly increased in the 7 days of the TSN I intervention group, the protein levels of PI3K and β-catenin, as well as p-PDK1/PDK1, p-AKT/AKT, and p-GSK3β/GSK3β protein ratios was significantly increased in the TSN I pretreatment groups [10]. Here, ITSN1 is linked to brain ischemia.