Given that NAFLD is accompanied by obesity, and considering that the known impacts of TLR4 signaling in high-fat-induced metabolic disarrangements (Liu et al., 2019; Zhang et al., 2020b), we therefore examined obesity-related parameters in HFD-fed mice in the presence of CLI-095 treatment. The gene discussed is TLR4; the disease is obesity due to melanocortin 4 receptor deficiency.