IDH1 and glioblastoma: We first developed a protocol to generate an in vitro vital patient-derived IDH1/2 wild-type GB 3D model that we termed “glioblastoma explant” (GB-EXP), which, unlike other models (6–8), is minimally handled, briefly grown in culture with no animal involvement, no dissociation and passaging to preserve the parental cytoarchitecture.