In addition, time-dependent ROC curves were used to compare the prognostic efficacy of PNI-GGT/ALT, PNI, GGT/ALT and AFP (Figure 2). Our results indicated that PNI-GGT/ALT had a higher AUC value (0.690) in predicting the 5- year OS of patients within the Milan criteria HCC after curative resection in comparison with AFP (0.501), PNI (0.634) and GGT/ALT (0.624). The gene discussed is AFP; the disease is hepatocellular carcinoma.