GZMB and neoplasm: It is worth mentioning that the inherent amount of tumor specific GrB+/IFNγ+ immune cells in lymph nodes of CT26 tumor bearing mice was significantly higher (987.17 ± 83.88) than in lymph nodes of B16F10 tumor bearing mice model (58.33 ± 28.19) as evident from the representative image of the stimulated control FluoroSpot wells (Figure 4).