110 of the 281 mutations show a potential functional impact according to wANNOVAR [48] and eight of these occurred in known cancer-related genes (PPARG, BRCA1, GATA1, ACR, WHSC1, FAT1, POLE and HOXD11) as reported in the cancer gene census [49] (Additional file 7). This evidence concerns the gene POLE and cancer.