Analyzing sorted BM subpopulations from five individuals with CH and 6 distinct ASXL1 mutations (Fig. 4A), we found that overall, ASXL1 allelic burden in mature T-cells was significantly lower compared to progenitor cell fractions including CD34+ progenitors (p = 0.018), HSC (p = 0.012), CMP (p = 0.00041), and MEP (p = 0.0016). This evidence concerns the gene ASXL1 and cyclic hematopoiesis.