In this study, homozygote cases (TT) will not show the symptoms and they only have a 162 bp band, heterozygote cases (TC) show some of the symptoms or some light symptoms and they have 3 bands: 162, 100 and 62 but the homozygote cases (CC), show sever symptoms and they have 2 bands 100 and 62 bp which agrees with the Iraq’s study who reported that TSHR rs2268458 polymorphisms were associated with hypothyroidism15 and disagrees with the Lebanon’s study who reported that TSHR rs2268458 polymorphisms were not associated with hypothyroidism and hyperthyroidism26. The gene discussed is TSHR; the disease is hypothyroidism.