To investigate the roles of SNHG1 in NB, we utilized the CRISPR/Cas9 system to generate SNHG1-depletion stable-clone SK-N-BE(2)C cells by transfecting guide RNAs targeting SNHG1 genomic loci (sgSNHG1) (Fig. 1A). The gene discussed is SNHG1; the disease is neuroblastoma.