CD4 and neoplasm: Using in situ hybridization, we corroborated these results finding not only the presence of CD3+CD4+SOCS3+ T cells in the human BTC tissue sections, but also their co-localization with HAVCR2+SPP1+CD68+ cells in the tumor microenvironment, demonstrating that HAVCR2+SPP1+CD68+ macrophages are located closer to CD3+CD4+SOCS3+ T cells than non-SPP1/HAVCR2+CD68+ macrophages (p = 0.04) (Figures 5J and S7C–S7F).