Historical research uncovers polymorphisms of UGT1A1 in the promoter or exon 1 regions contribute to unconjugated hyperbilirubinemia, which may be the source of gallstone formation, with UGT1A1*6 (c.211G>A, rs4148323, p.Gly71Arg), UGT1A1*27 (c.686C>A, rs35350960, p.Pro229Glu), UGT1A1*28 ([TA]6>[TA]7, rs3064744, rs4124874), and UGT1A1*60 (c.-3279T>G) mostly reported [26,27,28,29]. This evidence concerns the gene UGT1A1 and gallstones.