The prognosis of patients with AIS at 90-days after IVT was used as the dependent variable, and age, smoking, hypertension, diabetes, coronary artery disease, hyperlipidemia, atrial fibrillation, IL-6, MMP-9, ADAMTS13, TRX, TNC, and GSN were considered independent variables. The gene discussed is MMP9; the disease is diabetes mellitus.