Similarly, we observed lower Ocln on the mRNA (Fig. 3c) and protein levels in the diabetic vs non-diabetic mice, with the protein localized in the upper superficial umbrella cell layers of the bladder (Fig. 3d), 24 h (Fig. 3e, f) and 7 days (Fig. S2a, b) post E. coli infection. Here, OCLN is linked to escherichia coli infection.