As a first step to validate our selected candidates, we generated individual PLAC8 and SPNS1 knockout (KO) Calu1ACE2 cell lines using three different sgRNAs for each of these genes and infected them with S‐typed lentiviruses carrying a ZsGreen‐expressing plasmid to allow assessment of infection efficiency by flow cytometry (Fig 2A). The gene discussed is PLAC8; the disease is infection.