In this study, it was confirmed that miR-619-5p was the direct target gene of BCYRN1, further elucidating that miR-619-5p specifically targeted the CUE domain of protein 2 (CUEDC2), while BCYRN1/miR-619-5p inhibited glioma by inactivating the PTEN/AKT/p21 pathway in a CUEDC2-dependent manner. This evidence concerns the gene CUEDC2 and central nervous system cancer.