TJP1 and hepatocellular carcinoma: In this work, we found that ZO-1 was redistributed in extruded Huh7 cells by IF assays, and the expression of ZO-1 was increased in the cytoplasm of extruded HCC cells, meanwhile, F-actin staining intensity between the extruded cells and neighboring cells was significantly enhanced, suggesting that the redistribution of ZO-1 might be associated with the extrusion of HCC cells (Fig. 6A).