GBA1 and hepatocellular carcinoma: To investigate the function of GBA in HCC, two human HCC cell lines, HepG2 and MHCC-97H, which were demonstrated to have high GBA expression (Supplementary Fig. 1a), were transfected with three si-GBA plasmids (si-GBA-313, si-GBA-1255 and si-GBA-1620) to reduce GBA expression.