BANF1 and hepatocellular carcinoma: It is worth noting that the protein expression levels of LC3B and SQSTM1/p62 were significantly increased in HCC cells with si-GBA transfection, and these levels were further increased by combination treatment with si-GBA transfection and the late-stage autophagy inhibitor BAF compared to those in cells with si-GBA transfection or BAF treatment alone (LC3B protein, all P < 0.05 when using two si-GBA plasmids; SQSTM1/p62 protein, all P < 0.05 when using the si-GBA-313 plasmid, Fig. 2d).