DMD and Duchenne muscular dystrophy: Our ability to identify cell composition and gene expression profiles in nuclei prepared from very small (3 mg) samples of frozen human muscle now make feasible snRNASeq analysis on human remnant muscle tissue obtained in the context of dystrophin replacement or other DMD therapies, natural history studies, or from small core needle biopsies of unusual cases, enabling exploration of therapeutic and naturally occurring mechanisms of genetic repair and pathogenesis in DMD and other muscle conditions.