To investigate the role of ALS-causing TDP-43 mutations on TDP-43 mobility, we engineered TDP-43Halo constructs bearing either one mutation in the alpha-helical structure (M337V)14,31 and another mutation in the Glycine-/Serine-rich domain (A382T)14,32 (Supplementary Figs. 10, 11). The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.