However, it is important to note that the MK-2206 biochemical IC50 for AKT2 is 12 nM [40], which is 1000-fold higher than 12 μM of 4j, MK-2206 was expected to exhibit very high potency in glioma where AKT expression and signalling pathway are amplified [10]. Here, AKT1 is linked to glioma.