As shown in Figure 5(c), MVECs uptake of CFNs reduced following 1 h of pretreatment with different concentrations of the P-selectin inhibitor, and the uptake decreased by ~44% after the addition of 2 μM of the inhibitor, whereas it was reduced by ~12% for inhibitor concentration of 0.5 μM. P-selectin expression in ECs mimicking the endothelial microenvironment in atherosclerosis was further assessed by western blotting of proteins from CFN-tagged MVECs. Here, SELP is linked to atherosclerosis.